ABSTRACT
Silymarin, an extract from Silybum marianum, has been shown to have antioxidant properties. However, there is no scientific report on wound healing activity of the silymarin. The purpose of this study was to evaluate the effect of topical administration of silymarin on excision wound healing in rats. Excision wounds were made on the back of rats. Rats were divided into three groups, as control, vehicle, and treatment. Vehicle and treatment groups received polyethylene glycol and silymarin dissolved in polyethylene glycol, respectively. The control group did not receive any treatment. The wound tissues were removed on 5th, 10th and 15th day for histopathological analysis and total collagen determination by hydroxyproline assay. Results showed that silymarin increased epithelialization and decreased inflammation but did not have any effect on percentage of wound contraction, collagenization and hydroxyproline levels. It was concluded that silymarin can significantly stimulate epithelialization and reduce inflammation in full-thickness wounds in rats.
ABSTRACT
The present study was conducted to investigate the histological changes and wound healing effect of aqueous extract of Elaeagnus angustifolia. After creating full-thickness skin wounds on the back of 45 male Sprague-Dawley rats they were randomly divided into three groups. Treated group received the extract, positive control group were treated with mupirocin ointment 2% and control group did not receive any treatment. Wound healing rates were calculated on days 3, 5, 8, 10, 12 and 15 post-wounding and the wound tissues were harvested at 5, 10, and 15 days for histological analysis and hydroxyproline content measurement. The results indicated a significant increase in the percentage of wound contraction and hydroxyproline content in the treated group comparing to the control and positive control groups. A significant increase in the assigned histological scores was observed at 10 and 15 days in the treated and positive control groups compared to the control group. The results demonstrate that aqueous extract of Elaeagnus angustifolia accelerates cutaneous wound healing, and its effect may be due to the increased reepithelialization and collagen deposition in wound and so it can be considered as a therapeutic agent for wound healing.
ABSTRACT
Class-Pi of glutathione s-transferases [GST-Pi] is the specific form of GSTs that are known to participate particularly in the mechanisms of resistance to drugs and carcinogens. This class of the enzyme is referred to as class-P or class-Pi or class pi. The accepted terminology in this review article is class-Pi. In this article following a brief description of identified molecular forms of GSTs, we focus on GST-Pi. We review new findings about the structure and regulation of GST-Pi gene. Then, the role of GST-Pi in liver damage, oxidative stress, carcinogenesis and drug resistance are discussed. Also, the presence of common genetic polymorphism, hypermethylation in GST-Pi gene and the consequences GST-Pi knock out is regarded